Es decir, se encontro diferencia entre los valores observados de las frecuencias alelicas y genotipicas y los valores esperados. El genotipo II fue mas frecuente. TERMIUM® is the Government of Canada’s terminology and linguistic data bank. Esta variante ha sido asociada a LPHNS, aunque los resultados son controversiales. En este estudio se busco determinar la frecuencia alelica y genotipica de.
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The high polymorphism of the HLA system allows its typification to be used as valuable tool in establishing association to various illnesses, immune and genetic profiles; it also provides a guide to identifying compatibility among donors and receptors of organs transplants. The results obtained provide a useful reference framework for the population studied, allowing compatibility probability calculations to be performed for organ transplants.
The human leukocyte antigen system HLA of the major histocompatibility complex MHC contains a numerous family of genes located on the short arm of the 6th chromosome 6p These antigens are expressed in autosomal and codominant manner and they are inherited from both parents, which is why each individual expresses an antigen of paternal origin and one of maternal origin for each of the loci 1.
The high polymorphism of the HLA system and advancements in molecular biology have permitted for the typifying of these antigens to become important tools in establishing crecuencia of susceptibility or resistance to disease, development of tumor processes; playing an important role in understanding the variability of the immune response and determinant on the survival of organ or tissue transplants 2 – 8.
Because the allele frequencies and the disequilibrium patterns vary within a given population, the characterization of these molecules and the determination of their frequencies gain much pertinence and permits establishing parameters of association to disease in the frecuehcia, which has a predictive value in managing self-immune diseases like Systemic Erythematosus Lupus SEL and Rheumatoid Arthritis RAalso becoming frecuenncia important tool for the distribution of organs donated in the different transplant groups in the National Donation Network, as well as having an additional usefulness in estimating times the receptors must remain in the waiting list 9 Most of the Latin American populations studied are of indigenous origin or barely mixed 11 – The population treated in the Molecular Biology laboratory at Clinica Colsanitas SA for HLA typifying comes from different Colombian cities, offering an opportunity to determine the allele, genotype, alelca haplotype frequencies in patients of diverse origins.
This is a retrospective descriptive study, which included patients treated in the cities of Bogota, Cali, Barranquilla, Medellin, Villavicencio, Armenia, Bucaramanga, and Neiva. The variables of interest for this study were: Procedure of HLA typifying. Proportions were calculated to summarize the qualitative variables. The normality of the age freckencia was evaluated by using the Shapiro-Wilk test and summarized with a mean.
Homologation of the degree of resolution of the typifying to two digits was done on the data base for further analysis of allele, genotype, and haplotype frequencies The p values lower than or equal to 0. The genotipic, genotype, and haplotype frequencies and those corresponding to demographic characteristics were calculated in the Stata 9. This study used data from patients whose samples had already been taken and processed; hence, there was no additional alslica.
Moran Moguel, Maria Cristina [WorldCat Identities]
The confidentiality of the information was strictly protected. The samples had been taken after obtaining signed informed consent from the patients or their families and through medical request. Of the total number of patients included in the study, we obtained information on the place of birth from patients; who came from 28 departments in Colombia, with a greater representation from Cundinamarca, followed by Boyaca, Santander, Huila, Atlantico, and Cesar.
The population distribution per department can be seen in Table alelicx. Of the individuals included, Within the population analyzed, there were patients who frecuenciaa outpatient consultation to have the sample taken, as well as patients who were under pre-transplant study by the group at Clinica Colsanitas SA and cadaveric donors from the 1st regional facilities of the transplant network typified by the renal transplant group at Clinica Reina Sofia.
The genotypes revealing the highest frequencies for the HLA B locus were: It was possible to determine the level of the A locus for 19 alleles, grnotipica to those reported by Ossa et al. The analyses made by Ossa et al. The results obtained in this study correlate to those previously reported for a Latin American population, where the alleles with the greatest frequency were A02, A24, A01, and A68 1415 Only 28 alleles were identified for the B locus; while in prior studies conducted in Colombian populations, 43 alleles were reported for this locus 9 This marked difference is attributable to the resolution of the technique used in typifying HLA in our study, given that is some instances such resolution did not permit differentiation of the B15, B14, and B40 splits in the allelic variants currently considered.
Of the total population typified as B14 6. For this allele, we were not able to identify in all typifying events the specificity B62, B63, B70, B71, B72, and B75, because of the resolution of the kit used in typifying. Thus, the B15 frequency we found is higher than that reported in previous studies in Colombia. As well as for the B14 and B15 alleles, for the B40 allele we were not able to differentiate between the B61 and B60 alleles; hence, the frequency found for B40 is a reflection of the 3 alleles B40, B60, and B When comparing our results to the study with the population in the city of Bogota published by Ossa et al.
The results of the current study correspond mostly to that previously reported for Colombia, where we found a typical component of Latin population with an important Caucasian influence; nevertheless, the differences evidence the variations in the size and origin of the population studied, as well as the important influence of the resolution of the technique used in typifying. Given the heterogeneity of the population analyzed in the current study, it is important to keep the following aspects in mind: For this reason, more than having healthy individuals or patients in the population the origin does set an important relevant factor with respect to the distribution of frequencies.
Conducting this study permitted us to know the genotype, allele, and haplotype frequencies of the population analyzed and offers us useful tools to establish probabilities of finding compatible donors for patients in waiting lists for renal transplant. Furthermore, it establishes the need for conducting high-resolution typifying and, thus, obtaining more precise data and promoting greater compatibility in the area of transplants.
None of the authors has conflicts of interest related to this study. The haplotype structure of the human major histocompatibility complex.
Clinical features and prognosis of HLA-B27 positive and negative anterior uveitis in a Korean population. J Korean Med Sci.
Moran Moguel, Maria Cristina
Benjamin A, Bradley T. Pronostic assays for rejection and tolerance in organ transplantation. Association of human leukocyte antigen haplotypes with posttransplant lymphoproliferative disease after solid organ transplantation.
Alfaro E, Dipierri, J. The HLA dictionary Raymond M, Rousset F. Analysis of the frequencies of HLA-A, B, and C alleles and haplotypes in the five major ethnic groups of the United States reveals high levels of diversity in these loci and contrasting distribution patterns in these populations. HLA gene and haplotype frequencies in the North American population: